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Li Z, Zhang X, Hu X, Tian J, Kang H, Guo D, Liu J, Qu L. Development of an indirect ELISA assay for detecting antibodies against mammalian reovirus in pigs. J Virol Methods. 2018 Jul 14

发布日期:2018-07-24 17:39  浏览次数:

Development of an indirect ELISA assay for detecting antibodies against mammalian reovirus in pigs.
Li Z, Zhang X, Hu X, Tian J, Kang H, Guo D, Liu J, Qu L.
J Virol Methods.  2018 Jul14. pii:S0166-0934(17)30593-1.doi: 10.1016/j.jviromet.2018.07.008

Abstract
Mammalian reovirus (MRV) infects many species. Over the past decades, MRV infections in pigs have been reported, and several highly pathogenic MRV strains have recently been isolated in the United States. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) against the σ1 protein from a serotype 3 reovirus strain (MPC/04) was established to detect antibodies in pigs. The assay did not react with antisera against other pig pathogens and was consistent with the indirect immunofluorescence assay (IFA) and virus neutralization test (VNT). In conclusion, the assay is specific and highly sensitive, providing a method for large-scale monitoring of the serotype 3 MRV infection epidemiology in pigs.

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