Yue Jia, Changqi Hao, Qin Yang, Wanjiang Zhang, Gang Li, Siguo Liu, Xin Hua
Res Vet Sci.2021 Jun 5;138:62-68.doi: 10.1016/j.rvsc.2021.06.004. Online ahead of print.
Haemophilus parasuis is the main agent of Glässer's disease, which causes substantial losses in pig production. However, the pathogenic mechanism and virulence factors of H. parasuis have not been fully determined. In this study, berberine is shown to have a good therapeutic effect in vivo against H. parasuis; the minimal inhibitory concentration (MIC) in vitro was 2 μg/mL. Berberine inhibited H. parasuis adhesion to and invasion of PK-15 pig kidney cells. Proteomics studies of H. parasuis after berberine treatment identified a total of 97 differentially-expressed proteins; 35 upregulated and 62 downregulated. Bioinformatics analysis showed that berberine may inhibit the growth of H. parasuis by affecting outer membrane proteins, transferrins, and energy metabolism. This study provides a basis for the development of new antibacterial agents.
Keywords: Antibacterial mechanism; Bacteriostatic activity; Berberine; Haemophilus parasuis; Proteomics; Virulence factor.
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