Mengmeng Xu , Mengmeng Huang , Guodong Wang , Jingzhe Han , Xinxin Niu , Jinze Han , Hangbo Yu , Yulong Zhang , Runhang Liu , Ziwen Wu , Hongyu Cui , Yanping Zhang , Suyan Wang , Yongzhen Liu , Yuntong Chen , Yulu Duan , Liuan Li , Yulong Gao , Xiaole Qi

Poult Sci.2025 Jan 11;104(2):104807.doi: 10.1016/j.psj.2025.104807. Online ahead of print.

Abstract

Pro-IL-1β is an important inflammatory factor and is also a biomarker for detecting early pro-inflammatory immune responses. However, commercially available antibodies against chicken inflammatory factors are lacking, which prohibits an in-depth exploration of the mechanism of chicken inflammation. This study cloned and expressed chicken pro-IL-1β, and developed a hybridoma cell line 1E12 capable of stably secreting chicken pro-IL-1β monoclonal antibody (mAb). The secreted mAb 1E12 can recognize exogenous or endogenous chicken pro-IL-1β by Western blot and IFA techniques, and for the first time, a novel antigen epitope 13SSLSEETFY21 of chicken pro-IL-1β recognized by this mAb was identified. This study not only provides an important tool for the detection and research of chicken pro-IL-1β, but also has significant implications for understanding the antigenic structure and biochemical characteristics of chicken pro-IL-1β.

Keywords: Antigenic epitope; Chicken; Detection; Monoclonal antibody; Pro-IL-1β.