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A Sandwich ELISA for Quality Control of PCV2 Virus-like Particles Vaccine.Vaccines (Basel).2022 Dec 18;10(12):2175.doi: 10.3390/vaccines10122175

Mingxia Sun,Shanghui Wang,Zheng Fang,Man Zhao,Yanfei Gao,Tongqing An,Yabin Tu,Haiwei Wang,Xuehui Cai


Vaccines (Basel).2022 Dec 18;10(12):2175.doi: 10.3390/vaccines10122175.


Abstract

Porcine circovirus type 2 (PCV2) is a highly prevalent virus in pig farms worldwide that causes significant economic losses in the swine industry. The PCV2 virus-like particles (VLPs) are potent subunit vaccines that are widely used. Currently, the adopted quality control of VLPs vaccines is mainly based in animal testing, the titration of neutralizing antibodies, or other biochemical/biophysical assays. In this study, we generated a monoclonal antibody that can distinguish assembled PCV2 VLPs from the capsid proteins. Subsequently, a convenient Sandwich ELISA was developed based on the monoclonal antibody (mAb) that recognizes the PCV2 VLPs specifically. This assay can be used for the quantity and quality control of PCV2 VLPs vaccines for both the intermediate or final products with high accuracy.


Keywords: ELISA; PCV2; VLPs; quality control; vaccine.


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